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B.STU.1906 - Daniel Forwood Postgraduate scholarship

MLA supports under/postgraduates to work on industry’s complex problems by providing opportunities for them to enter the red meat industry.

Project start date: 28 July 2019
Project end date: 29 June 2022
Publication date: 17 January 2024
Project status: Completed
Livestock species: Grain-fed Cattle, Grass-fed Cattle, Sheep, Goat, Lamb
Relevant regions: National, International

Summary

This study characterised the nutritive and microbial profiles and the fermentation characteristics of silage with the following compositions on a dry matter (DM) basis: (1) 100% sorghum, (2) 70% sorghum + 30% carrot or pumpkin, and (3) 40% sorghum + 60% carrot or pumpkin. The treatments were further divided based on the addition or no addition of a probiotic inoculant. After 70 days of ensiling, the silage was incubated for 48h using the in vitro batch culture technique. Crude protein and non-fiber carbohydrates in the silage increased (P ≤ 0.01) by 5.7 percent point (pp) and 9.6 pp, respectively, with pumpkin at 60% DM. The V4 region of the 16S rRNA gene was sequenced to profile pre-ensiled and ensiled archeal and bacterial communities. Silages containing carrot or pumpkin strongly influenced the microbial structure (PERMANOVA: R2 = 0.75; P < 0.001), despite the ensiled treatments being dominated by Lactobacillus spp., except for the control, which was dominated by Weissella and Pediococcus spp. (P < 0.01). Linear discriminant analysis indicated that carrot and pumpkin silages were responsible for the increased relative abundance of Lactobacillus and Acinetobacter spp. (log LDA score ≥ 2), respectively. After 48h of incubation, carrot and pumpkin inclusion increased (P < 0.01) the in vitro DM digestibility by 22.5 and 31.3%, increased the total volatile fatty acids (VFAs) by 16 and 20.6% (P < 0.01), respectively, and showed a tendency (P = 0.07) to increase the gas production. Therefore, this study supports the use of carrot or pumpkin in sorghum silages to maximise feed digestibility and total VFA concentrations.

Objectives

Characterisation of the nutritive and microbial profiles and the fermentation characteristics of silage with the following compositions on a dry matter (DM) basis: (1) 100% sorghum, (2) 70% sorghum + 30% carrot or pumpkin, and (3) 40% sorghum + 60% carrot or pumpkin.

Key findings

The study supports the use of carrot or pumpkin in sorghum silages to maximise feed digestibility and total volatile fatty acid concentrations.

Benefits to industry

Investigation of unsalable vegetables as alternative ruminant feeds.

MLA action

Industry support.

Future research

MLA will continue supporting under/postgraduates to work on industry’s complex problems by providing opportunities for them to enter the red meat industry.

For more information

Contact Project Manager: Joshua Whelan

E: reports@mla.com.au