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Cysticercus bovis - Enhanced classification of suspect lesions identified at meat inspection

Project start date: 01 February 2011
Project end date: 16 August 2013
Publication date: 01 July 2012
Project status: Completed
Livestock species: Sheep, Lamb, Grassfed cattle, Grainfed cattle
Relevant regions: National
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Summary

A sensitive and specific probe-based molecular assay has been developed and evaluated to improve detection of bovine cysticercosis caused by Taenia saginata in slaughtered Australian beef cattle. Two real-time PCR approaches, including a SYBR green dye and Taqman MGB hybridisation probe assay, were compared for their diagnostic utility. The Taqman MGB probe assay was selected for further test performance and validation studies based on its fast turnaround time, ease of interpretation and high levels of specificity and sensitivity.

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Project manager: Khanh Huynh
Primary researcher: University of Queensland